P.C. Shannon

Ok, I don’t actually hate science, but I certainly feel that way sometimes when doing experiments. Here’s an example. In my system, I have some RNA (an aptamer, in fact) that has been labeled with a fluorescent dye. I also have a shorter piece of DNA that is complimentary to the aptamer, so it will bind to the aptamer. The DNA is labeled with a quencher that prevents the dye from emitting its signal. Basically, when the DNA is present, the aptamer doesn’t glow, and when the DNA is absent, it does glow.

At least, that’s the way it should work. In some of my experiments, the more DNA I added, the brighter the aptamer glowed. This is the exact opposite of what should happen. It’s like the TV turning off when you press the on switch, only you can’t blame it on faulty wiring. Maybe it’s more like water flowing uphill, since these are basic physical properties of matter we’re talking about.

Nonetheless, it’s very frustrating. Fortunately, that project was canceled recently, due in part to the confounding results I was getting.

I’ve been having some trouble getting back on track with my other projects. I’m not sure why, exactly. Sometimes simply having to do something makes it unappealing, no matter how interesting it might be if it weren’t a requirement.

But in the last few days, I’ve felt really on top of things. I’ve been very productive. I worked on my mountain bike and have gone on a few short rides. I’ve taken care of various projects around the house. I’ve even made some progress on my main research project. Hopefully, I can keep things on track and maintain my forward momentum.

Plus, I always have Piled Higher and Deeper to console me in any event.